rabbit polyclonal vegf-147 antibody  (Santa Cruz Biotechnology)

Journal: Journal of Biological Chemistry

Article Title: Transforming Growth Factor-β1 Stimulates Vascular Endothelial Growth Factor 164 via Mitogen-activated Protein Kinase Kinase 3-p38α and p38δ Mitogen-activated Protein Kinase-dependent Pathway in Murine Mesangial Cells

doi: 10.1074/jbc.m403758200

Figure Lengend Snippet: FIG. 3. Time course of TGF-1-stimulated VEGF protein syn- thesis in murine mesangial cells. Wild-type mouse mesangial cells were incubated in the absence (Ctl) or in the presence of exogenous TGF-1 (2 ng/ml) for the indicated time periods. Total cell lysates were isolated and subjected to Western blot analyses using polyclonal anti- VEGF antibodies, as described under “Experimental Procedures.” As loading controls, the same cell lysates were subjected to immunoblot- ting with mouse monoclonal anti--tubulin antibodies.

Article Snippet: VEGF (147) rabbit polyclonal antibodies and -tubulin antibodies were obtained from Santa Cruz Biotechnology.

Techniques: Incubation, Isolation, Western Blot

Journal: Journal of Biological Chemistry

Article Title: Transforming Growth Factor-β1 Stimulates Vascular Endothelial Growth Factor 164 via Mitogen-activated Protein Kinase Kinase 3-p38α and p38δ Mitogen-activated Protein Kinase-dependent Pathway in Murine Mesangial Cells

doi: 10.1074/jbc.m403758200

Figure Lengend Snippet: FIG. 4. Inhibition of TGF-1-stimulated VEGF gene expression in MKK3-deficient murine mesangial cells. Total RNA isolated from wild-type (Mkk3/) and MKK3-deficient (Mkk3/) mouse me- sangial cells incubated in the absence (Ctl) or in the presence of exog- enous TGF-1 (2 ng/ml) for the indicated time periods were subjected to Northern blot hybridization with 32P-labeled cDNA probe correspond- ing to VEGF. 18 S rRNA hybridization signals served as normalization for RNA loading.

Article Snippet: VEGF (147) rabbit polyclonal antibodies and -tubulin antibodies were obtained from Santa Cruz Biotechnology.

Techniques: Inhibition, Gene Expression, Isolation, Incubation, Northern Blot, Hybridization, Labeling

Journal: Journal of Biological Chemistry

Article Title: Transforming Growth Factor-β1 Stimulates Vascular Endothelial Growth Factor 164 via Mitogen-activated Protein Kinase Kinase 3-p38α and p38δ Mitogen-activated Protein Kinase-dependent Pathway in Murine Mesangial Cells

doi: 10.1074/jbc.m403758200

Figure Lengend Snippet: FIG. 1. Effect of deletion of Mkk3 on TGF-1 induced gene expression in murine mesangial cells. cDNA microarray analysis of the relative levels of gene expression in from wild-type (Mkk3/) and MKK3-deficient (Mkk3/) mouse mesangial cells treated with exoge- nous TGF-1 (2 ng/ml) for 6 h. 32P-Labeled cDNA probes generated from reverse transcription of DNase-treated RNA were hybridized to the AtlasTM mouse 1.2 arrays containing 1176 mouse genes (Clontech) as described under “Experimental Procedures.” The position of VEGF cDNA is indicated by the arrow. The relative expression of housekeep- ing genes served to normalize gene expression levels, and one (glycer- aldehyde-3-phosphate dehydrogenase) is indicated by the arrowhead.

Article Snippet: VEGF (147) rabbit polyclonal antibodies and -tubulin antibodies were obtained from Santa Cruz Biotechnology.

Techniques: Gene Expression, Microarray, Labeling, Generated, Reverse Transcription, Expressing

Journal: Journal of Biological Chemistry

Article Title: Transforming Growth Factor-β1 Stimulates Vascular Endothelial Growth Factor 164 via Mitogen-activated Protein Kinase Kinase 3-p38α and p38δ Mitogen-activated Protein Kinase-dependent Pathway in Murine Mesangial Cells

doi: 10.1074/jbc.m403758200

Figure Lengend Snippet: FIG. 2. Stimulation of VEGF mRNA expression by TGF-1 in murine mesangial cells. Wild-type mouse mesangial cells were incu- bated in the absence (Ctl) or in the presence of exogenous TGF-1 (2 ng/ml) for the indicated time periods. Total RNA was extracted and subjected to Northern blot hybridization with 32P-labeled cDNA probe corresponding to VEGF, as described under “Experimental Procedures.” 18 S rRNA hybridization signals served as normalization for RNA loading.

Article Snippet: VEGF (147) rabbit polyclonal antibodies and -tubulin antibodies were obtained from Santa Cruz Biotechnology.

Techniques: Expressing, Northern Blot, Hybridization, Labeling

Journal: Journal of Biological Chemistry

Article Title: Transforming Growth Factor-β1 Stimulates Vascular Endothelial Growth Factor 164 via Mitogen-activated Protein Kinase Kinase 3-p38α and p38δ Mitogen-activated Protein Kinase-dependent Pathway in Murine Mesangial Cells

doi: 10.1074/jbc.m403758200

Figure Lengend Snippet: FIG. 5. Effects of MKK3 deficiency on TGF-1-stimulated pro- tein synthesis of VEGF isoforms, VEGF164 and VEGF188, in mu- rine mesangial cells. Total cell lysates from wild-type (Mkk3/) and MKK3-deficient (Mkk3/) mouse mesangial cells incubated in the absence () or in the presence () of exogenous TGF-1 (2 ng/ml) for 24 h were subjected to Western blot analyses using polyclonal anti- VEGF antibodies, as described under “Experimental Procedures.” As loading controls, the same cell lysates were subjected to immunoblot- ting with mouse monoclonal anti--tubulin antibodies.

Article Snippet: VEGF (147) rabbit polyclonal antibodies and -tubulin antibodies were obtained from Santa Cruz Biotechnology.

Techniques: Incubation, Western Blot

Journal: Journal of Biological Chemistry

Article Title: Transforming Growth Factor-β1 Stimulates Vascular Endothelial Growth Factor 164 via Mitogen-activated Protein Kinase Kinase 3-p38α and p38δ Mitogen-activated Protein Kinase-dependent Pathway in Murine Mesangial Cells

doi: 10.1074/jbc.m403758200

Figure Lengend Snippet: FIG. 6. Inhibition of TGF-1-stimulated the VEGF164 isoform by dominant negative mutants of p38 and p38 in murine me- sangial cells. Wild-type mouse mesangial cells transfected with empty vector (pcDNA3.1), dominant negative mutant of p38 (p38 dnm), or dominant negative mutant of p38 (p38 dnm), were incubated in the absence () or in the presence () of exogenous TGF-1 (2 ng/ml) for 24 h. Total cell lysates were isolated and subjected to Western blot anal- yses using polyclonal anti-VEGF antibodies, as described under “Ex- perimental Procedures.” As loading controls, the same cell lysates were subjected to immunoblotting with mouse monoclonal anti--tubulin antibodies.

Article Snippet: VEGF (147) rabbit polyclonal antibodies and -tubulin antibodies were obtained from Santa Cruz Biotechnology.

Techniques: Inhibition, Dominant Negative Mutation, Transfection, Plasmid Preparation, Incubation, Isolation, Western Blot

Journal: Journal of Biological Chemistry

Article Title: Transforming Growth Factor-β1 Stimulates Vascular Endothelial Growth Factor 164 via Mitogen-activated Protein Kinase Kinase 3-p38α and p38δ Mitogen-activated Protein Kinase-dependent Pathway in Murine Mesangial Cells

doi: 10.1074/jbc.m403758200

Figure Lengend Snippet: FIG. 7. Effects of SB203580 on TGF-1-stimulated protein syn- thesis of VEGF isoforms, VEGF164 and VEGF188, in murine me- sangial cells. Wild-type mouse mesangial cells, pretreated without () or with 10–30 M SB203580, were incubated in the absence () or in the presence () of exogenous TGF-1 (2 ng/ml) for 24 h. Total cell lysates were isolated and subjected to Western blot analyses using polyclonal anti-VEGF antibodies, as described under “Experimental Procedures.” As loading controls, the same cell lysates were subjected to immunoblotting with mouse monoclonal anti--tubulin antibodies.

Article Snippet: VEGF (147) rabbit polyclonal antibodies and -tubulin antibodies were obtained from Santa Cruz Biotechnology.

Techniques: Incubation, Isolation, Western Blot

Journal: Journal of Biological Chemistry

Article Title: Transforming Growth Factor-β1 Stimulates Vascular Endothelial Growth Factor 164 via Mitogen-activated Protein Kinase Kinase 3-p38α and p38δ Mitogen-activated Protein Kinase-dependent Pathway in Murine Mesangial Cells

doi: 10.1074/jbc.m403758200

Figure Lengend Snippet: FIG. 8. Cooperative inhibition of TGF-1-stimulated VEGF164 isoform in murine mesangial cells carrying dominant negative mutant p38 by SB 203580. Wild-type mouse mesangial cells transfected with empty vector (pcDNA3.1) or dominant negative mutant of p38 (p38 dnm) were pretreated without () or with SB203580 (30 M) and incubated in the absence () or in the presence () of exogenous TGF-1 (2 ng/ml) for 24 h. Total cell lysates were isolated and subjected to Western blot analyses using polyclonal anti-VEGF antibodies, as described under “Experimental Procedures.” As loading controls, the same cell lysates were subjected to immunoblotting with mouse monoclonal anti--tubulin antibodies.

Article Snippet: VEGF (147) rabbit polyclonal antibodies and -tubulin antibodies were obtained from Santa Cruz Biotechnology.

Techniques: Inhibition, Dominant Negative Mutation, Transfection, Plasmid Preparation, Incubation, Isolation, Western Blot

Journal: Journal of Biological Chemistry

Article Title: Transforming Growth Factor-β1 Stimulates Vascular Endothelial Growth Factor 164 via Mitogen-activated Protein Kinase Kinase 3-p38α and p38δ Mitogen-activated Protein Kinase-dependent Pathway in Murine Mesangial Cells

doi: 10.1074/jbc.m403758200

Figure Lengend Snippet: FIG. 9. Effects of recombinant mouse VEGF164 on the expression of pro-1(I) collagen, fibronectin, and PAI-1 in murine mesangial cells. Total RNA isolated from wild-type mouse me- sangial cells incubated in the absence (Ctl) or in the presence of exogenous VEGF (100 ng/ml) for the indicated time periods were subjected to Northern blot hybridization with 32P-labeled cDNA probes corresponding to pro-1(I) collagen (Pro-1(I)Col), fibronectin (FN), and PAI-1. 18 S rRNA hybridization signals served as normalization for RNA loading.

Article Snippet: VEGF (147) rabbit polyclonal antibodies and -tubulin antibodies were obtained from Santa Cruz Biotechnology.

Techniques: Recombinant, Expressing, Isolation, Incubation, Northern Blot, Hybridization, Labeling